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Analysis of Street Drugs in Seized Material without Primary Reference Standards

Analysis of Street Drugs in Seized Material without Primary Reference Standards

  1. trptamene
    A novel approach was used to analyze street drugs in
    seized material without primary reference standards.
    Identification was performed by liquid chromatography/
    time-of-flight mass spectrometry (LC/TOFMS), essentially
    based on accurate mass determination using a target
    library of 735 exact monoisotopic masses. Quantification
    was carried out by liquid chromatography/chemiluminescence
    nitrogen detection (LC/CLND) with a single
    secondary standard (caffeine), utilizing the detector’s
    equimolar response to nitrogen. Sample preparation
    comprised dilution, first with methanol and further with
    the LC mobile phase. Altogether 21 seized drug samples
    were analyzed blind by the present method, and results
    were compared to accredited reference methods utilizing
    identification by gas chromatography/mass spectrometry
    and quantification by gas chromatography or liquid chromatography.
    The 31 drug findings by LC/TOFMS comprised
    19 different drugs-of-abuse, byproducts, and adulterants,
    including amphetamine and tryptamine designer
    drugs, with one unresolved pair of compounds having an
    identical mass. By the reference methods, 27 findings
    could be confirmed, and among the four unconfirmed
    findings, only 1 apparent false positive was found. In the
    quantitative analysis of 11 amphetamine, heroin, and
    cocaine findings, mean relative difference between the
    results of LC/CLND and the reference methods was 11%
    (range 4.2-21%), without any observable bias. Mean
    relative standard deviation for three parallel LC/CLND
    results was 6%. Results suggest that the present combination
    of LC/TOFMS and LC/CLND offers a simple solution
    for the analysis of scheduled and designer drugs in seized
    material, independent of the availability of primary reference
    standards.

    Anal. Chem. 2004, 76, 7375-7379