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Benzodiazepine receptor-dependent modulation of neutrophil (PMN) free amino- and a-keto acid profile

Benzodiazepine receptor-dependent modulation of neutrophil (PMN) free amino- and a-keto acid profile

  1. mersann
    Authors: J. Muhling, J. Gonter, K. A. Nickolaus, R. Matejec, I. D. Welters, M. Wolff, A. Sablotzki, J. Engel, M. Krull, T. Menges, M. Fuchs, M. G. Dehne, and G. Hempelmann

    Abstract: We have examined the effects of midazolam, Ro 5-4864 (agonist for ‘‘peripheral’’ [p] benzodiazepine receptors [BR]), PK 11195 (antagonist for pBR), flumazenil (antagonist for ‘‘central’’ BR), naloxone (antagonist for opiate receptors) and the combination of midazolam and Ro 5-4864, PK 11195, flumazenil or naloxone on intracellular amino- and -keto acids and the immune function markers superoxide anion (O2 ), hydrogen peroxide (H2O2) and released myeloperoxidase (MPO) activity in neutrophils (PMN). Only midazolam and Ro 5-4864 led to significant changes in the dynamic PMN free amino- and -keto acid pools. Concerning PMN immune function markers, midazolam and Ro 5-4864 significantly decreased O2 À and H2O2 formation and released MPO. When midazolam and Ro 5-4864 were applied together they appeared to act additively. Pre-incubation with PK 11195 partially neutralized the mid azolam effects whereas flumazenil or naloxone showed no effects. We therefore believe that pBR are involved in the signal transmission of anesthetic-induced cellular metabolic changes in PMN.