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Case report: Etizolam and its major metabolites in two unnatural death cases

Case report: Etizolam and its major metabolites in two unnatural death cases

  1. catseye
    Takuma Nakamae, Tatsuo Shinozuka, Chizuko Sasaki, Akira Ogamo, Chikako Murakami-Hashimoto, Wataru Irie, Masaru Terada, Shigeki Nakamura, Masataka Furukawa, Katsuyoshi Kurihara

    Forensic Science International
    Volume 182, Issue 1 , Pages e1-e6, 20 November 2008

    ABSTRACT:
    A simultaneous analytical method for etizolam and its main metabolites (a-hydroxyetizolam and 8-
    hydroxyetizolam) in whole blood was developed using solid-phase extraction, TMS derivatization and
    ion trap gas chromatography tandem mass spectrometry (GC–MS/MS). Separation of etizolam, TMS derivatives of a-hydroxyetizolam and 8-hydroxyetizolam and fludiazepam as internal standard was performed within about 17 min. The inter-day precision evaluated at the concentration of 50 ng/mL etizolam, a-hydroxyetizolam and 8-hydroxyetizolam was evaluated 8.6, 6.4 and 8.0% respectively. Linearity occurred over the range in 5–50 ng/mL. This method is satisfactory for clinical and forensic purposes.
    This method was applied to two unnatural death cases suspected to involve etizolam. Etizolam and its two metabolites were detected in these cases.