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D2 dopamine receptor-mediated inhibition of forskolin-stimulated adenylate cyclase activity in rat s

D2 dopamine receptor-mediated inhibition of forskolin-stimulated adenylate cyclase activity in rat s

  1. Calliope
    Neurosci Lett. 1985 Aug 30;59(2):177-82. [PMID: 2932659]

    Battaglia, George ; Norman, Andrew B. ; Hess, Ellen J. ; Creese, Ian

    Abstract
    Forskolin markedly stimulates striatal adenylate cyclase activity in a concentration-dependent manner, and at 10 −4 M produces an approximate 40-fold increase in enzyme activity above basal levels. Dopamine (in the presence of 100 nM SCH 23390), bromocryptine and quinpirole (LY 171555) significantly inhibit both basal and forskolin-stimulated adenylate cyclase activity. There is a significant increase in the absolute but not in the percent inhibition of enzyme activity by dopaminergic agonists as a function of forskolin concentration. This inhibition is agonist-concentration dependent and antagonized by the D 2 antagonist, spiperone. These results suggest that forskolin may be used as a tool for amplifying the abolute D 2 -receptor-mediated inhibition of adenylate cyclase in rat striatal homogenates.