1. Dear Drugs-Forum readers: We are a small non-profit that runs one of the most read drug information & addiction help websites in the world. We serve over 4 million readers per month, and have costs like all popular websites: servers, hosting, licenses and software. To protect our independence we do not run ads. We take no government funds. We run on donations which average $25. If everyone reading this would donate $5 then this fund raiser would be done in an hour. If Drugs-Forum is useful to you, take one minute to keep it online another year by donating whatever you can today. Donations are currently not sufficient to pay our bills and keep the site up. Your help is most welcome. Thank you.
    PLEASE HELP
    Dismiss Notice

Determination of phenylethylamines in hallucinogenic cactus species

By high-performance liquid chromatography with photodiode-array detection

  1. Calliope
    Journal of Chromatography, 623 (1992) 381-385

    Hans-J org Helmlin, Daniel Bourquin and Rudolf Brenneisen

    A high-performance liquid chromatographic procedure with photodiode-array detection has been developed to create phytochemical profiles of phenylalkylamine-containing cactus species. The basic methanolic cactus extracts with methoxamine as internal standard were separated on a 3-µm ODS column with acetonitrile-water-phosphoric acid-hexylamine as the mobile phase. Peak assignation was performed by on-line UV detection (190-300 nm) and by gas chromatography-mass spectrometry of the isolated compounds. The quantitation of mescaline was done at 205 nm. The excellent sensitivity (the detection limit of mescaline was 500 pg, corresponding to 0.002% in cactus material) allowed the analysis of milligram amounts of cactus material taken from living plants. The mescaline content of the psychotropic Peyote cactus Lophophora williamsii (Lem. ex Salm-Dyck) Coult. ranged from 680 to 1010 mg per 100 g. The morphologically very similar Lophophora diffusa (Croizat) Bravo could be differentiated by the absence of mescaline and the dominant alkaloid pellotine.