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Development of a Psilocybin Immunoassay for Serum and Blood Samples (Albers et al, 2004)

Development of a Psilocybin Immunoassay for Serum and Blood Samples (Albers et al, 2004)

  1. Jatelka
    Int J Legal Med (2004) 118: 326–331 DOI 10.1007/s00414-004-0469-9

    C. Albers, H. Köhler, M. Lehr, B. Brinkmann, J. Beike

    After the immunisation of rabbits with a psilocin-specific immunogen, polyclonal antisera were obtained. With these antisera a competitive, heterogeneous radioimmunoassay for the detection of psilocin was developed. As tracer a derivative of psilocin was synthesised, which contained a tritiated CH3 group. The antisera showed a specific reaction with psilocin. The cross-reactivity of structurally related endogenous sub-stances like serotonin, tryptophan and tyrosine was below 0.01%. Also common drugs of abuse (Δ9-tetrahydrocan-nabinol, cocaine, morphine, amphetamine) showed negli-gible cross-reactivity (0.01–2%). Only tricyclic neurolep-tics with a (dimethylamino)ethyl side-chain showed some cross-reactivity (20%). Spiked serum and blood samples were analysed with this new immunoassay and the results obtained were compared with the values measured with a validated GC-MS method.