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Simultaneous analysis of THC and its metabolites in blood using liquid chromatography–tandem mass

Simultaneous analysis of THC and its metabolites in blood using liquid chromatography–tandem mass

  1. Anonymous
    Maria del Mar Ramirez Fernandeza,∗, Gert De Boecka, MichelleWoodb,
    Manuel Lopez-Rivadullac, Nele Samyna

    Journal of Chromatography B, 875 (2008) 465–470

    Cannabis is considered to be the most widely abused illicit drug in Europe. Consequently, sensitive and
    specific analytical methods are needed for forensic purposes and for cannabinoid pharmacokinetic and
    pharmacodynamic studies. A simple, rapid and highly sensitive and specific method for the extraction and
    quantification of 9-tetrahydrocannabinol (THC), 11-hydroxy- 9-tetrahydrocannabinol (11-OH-THC)
    and 11-nor-9-carboxy- 9-tetrahydrocannabinol (THC-COOH) in blood is presented. The method was
    fully validated according to international guidelines and comprises simultaneous liquid–liquid extraction
    (LLE) of the three analytes with hexane:ethyl acetate (90:10, v/v) into a single eluant followedby separation
    and quantification using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Chromatographic
    separation was achieved using a XBridge C18 column eluted isocratically with methanol:0.1%
    formic acid (80:20, v/v). Selectivity of the method was achieved by a combination of retention time, and
    two precursor–product ion transitions. The use of the LLE was demonstrated to be highly effective and
    led to significant decreases in the interferences present in the matrix. Validation of the method was performed
    using 250L of blood. The method was linear over the range investigated (0.5–40g/L for THC,
    1–40g/L for 11-OH-THC, and 2–160g/L for THC-COOH) with excellent intra-assay and inter-assay precision; relative standard deviations (RSDs) were