KCA Lab report of Hexahydrocannabinol isomers sample

Study Author(s):

KCA Labs & Colorado Chromatography

Journal Name:

Hexahydrocannabinol HHC Packet

Publication Date:

July 2021

Must scroll down to bottom of the PDF to view the part with the lab report done by KCA labs on behalf of a company to lab test their claimed Hexahydrocannabinol product that reports there are 3 different isomers of HHC in the product but it is unknown exactly what isomers they are.

"GC-MS/MS Analysis of Sample #2132 The sample was subjected to GC-MS/MS analysis to obtain evidence for the presence of hexahydrocannabinol diastereomers and confirm the identity of the ∆8-THC. The GC/MS spectrum of hexahydrocannabinol was obtained from the NIST library and is shown below. Prominent ions are reported at m/z 193 (base peak), m/z 273, and m/z 260."

"Those ions characteristic of hexahydrocannabinol were monitored during the GC/MS analysis of Sample 2123. The selected ion chromatogram is shown below and the relative abundances of the ions characteristic of hexahydrocannabinol from the 1st prominent peak at about 6.08 min are shown in the table below"

"These results indicate with reasonable assurance that at least two and possibly more isomers of hexahydrocannabinol are present in the sample submitted for analysis. Proof of identity would require a reference standard"

"Conclusions
• Sample #2132 contains a small amount of D8-THC based on HPLC-PDA, LC-MS, and GC-MS data.
• Sample #2132 contains components characterized by greater lipophilicity than D8-THC based on longer HPLC and LC retention times. The components eluting after D8-THC are characterized by a pseudomolecular ion at m/z 317 based on positive ionization electrospray LC-MS analysis indicating an apparent molecular weight of 316, consistent with that of the hexahydrocannabinol isomers. Analysis of sample #2132 by GC/MS analysis indicated the presence of at least two substances with an apparent molecular mass of 316 and characterized by fragment ions at m/z 193, m/z 260, and m/z 273. These fragment ions are consistent with reported fragment ions of hexahydrocannabinol.
• Confirmation of the presence of hexahydrocannabinol isomers in sample #2132 requires availability of a reference standard.
• Sample #2132 contains no other detectable cannabinoids from our collection of more than forty reference standards. "

REPORT HAS NOT BEEN PEER REVIEWED FOR ACCURACY AND WAS DONE BY A HEMP LAB COMPANY ON BEHALF OF ANOTHER COMPANY.